Mcf 7 cell line pdf
Apoptotic efficacy of biogenic silver nanoparticles on human breast cancer MCF-7 cell lines MCF-7 cell lines at different concentrations (1.56, 6.25, 25 lL) involve intracellular suicide program possessing morphological changes like cell shrinkage, oxidative stress, coiling and biochemical response leading to apoptosis as shown in Fig. 4d–f. It is quite obvious from the results that
07/10/2002 · To investigate the mechanisms underlying apoptosis in breast cancer cells, staurosporine was used as an apoptotic stimulus in the human breast cancer cell lines MCF-7 and T47D. Staurosporine induced dose and time dependent increases in DNA fragmentation
MCF-7 is a commonly used breast cancer cell line, that has been promoted for more than 40 years by multiple research groups but its characteristics have never been gathered in a consistent review article. The current paper provides a broad description of the MCF-7 cell line, including the molecular profile, proliferation, migration, invasion
The MCF7/164R-7 Cell line is a breast cancer cell line resistant to fulvestrant. Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old Caucasian woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer Institute.
An optimized MCF-7 cell line transfection kit, the MCF-7 transfection kit, is available from Altogen Biosystems.. The transfection reagent is optimized for MCF-7 cells and enables a high efficiency transfer of miRNA, siRNA or plasmid DNA into MCF-7 cells.
Media in category “MCF-7 cells” The following 93 files are in this category, out of 93 total.
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26].It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].MCF-7 cells are useful for in vitro breast studies because they retained several ideal characteristics
by highly aggressive breast cancer cell lines, we aimed at elucidating their role on the less aggressive breast cancer cell line MCF-7, which does not secret these cytokines. Methods: The MCF-7 cell line was treated with either senescence-conditioned medium (SCM), IL-6 or IL-8 and then
NICEATM DRAFT VALIDATION STUDY REPORT: MCF-7 CP TM June 18, 2012 . 6 . 2.2 Materials 2.2.1 MCF-7:WS8 Cells . The MCF-7:WS8 cell line is derived from MCF-7 immortalized human breast adenocarcinoma cells and endogenously express both human ERα and ERβ. CCi provided cryopreserved MCF-7:WS8 cells from their cell bank to Hiyoshi and KFDA for the
resistant breast cancer cell line, MCF-7/Taxol, was established from the drug-sensitive parent cell line MCF-7. The biological properties of MCF-7/Taxol, including its drug resistance profile and profile of paclitaxel binding proteins, were analysed and compared with the parent cell line. A number of paclitaxel binding proteins were present in MCF-7 cells but absent from MCF-7/Taxol cells
11/09/2019 · However, this study showed that in addition to the energy metabolism pathways, it identified the Warburg effect as a key component in MCF-7 cell lines …
MCF-7 cells is able to process estrogen in the form of estradiol via estrogen receptors in the cell cytoplasm. This results in the MCF-7 cell line being an estrogen receptor (ER) positive cell line. MCF-7 is also progesterone receptor positive and HER2 negative. The MCF7 / GFP reporter cell line was transformed from the MCF7 cell line, and stably express the fluorescent enhanced GFP marker
Apoptotic efficacy of biogenic silver nanoparticles on Human breast cancer MCF-7 cell lines. Article (PDF Available) · November 2015 with 359 Reads How we measure ‘reads’
View MCF-7 cell line Research Papers on Academia.edu for free.
Journal of Research in Pharmacy Research Article www.jrespharm.com How to cite this article: Kan ST, Ahmad MS, Syed Abdul Aziz SS, Awang K, Mhd Bakri Y, Nafiah MA, Abbas P, Hashim YZHY. Oxoaporphine alkaloids from the barks of Platymitra siamensis Craib (Annonaceae) and their cytotoxicity against MCF-7 cancer cell line.
MCF-7 Breast Cancer Cell Line, a Model for the Study of th e Association Between Inflammation and ABCG2-Mediated Multi Drug Resistance 347 mucosa (Chan et al., 1995) and regulation of renal blood flow (Tanioka et al., 2003).
Cell line profile MCF7 (ECACC catalogue no. 86012803
MCF-7 Cell Line an overview ScienceDirect Topics
MCF-7 est le nom de la lignée de cellules tumorales mammaires la plus utilisée dans les laboratoires de recherche sur le cancer du sein. Ainsi, elle est mentionnée dans plus d’un tiers des articles scientifiques rapportant des travaux sur des lignées tumorales mammaires (voir PubMed).
Cell line profile MCF7 (ECACC catalogue no. 86012803) Cell line history . MCF7 was derived from the pleural effusion from a 69 year old female suffering from a breast adenocarcinoma1. It was named after the Michigan Cancer Foundation (MCF) and is the most studied human breast cancer cell line in the world2. Key characteristics
Origin of MCF-7 Cell Line. MCF-7 is a cell line that was first isolated in 1970 from the breast tissue of a 69-year old Caucasian woman. Of the two mastectomies she received, the first revealed that the removed tissue was benign.
01/06/1987 · MCF-7 human breast cancer cells are used widely for studies of tumor biology and hormone mechanism of action. Conflicting results have often been obtained in studies reported from different laboratories. In this report several biological properties were studied in four MCF-7 cell lines obtained from different laboratories. MCF-7 (ATCC), MCF-7, MCF-7 (KO), and MCF-7 (S) …
17/06/2015 · Dear all, I want to predict the IC50 values of my new synthesized compounds verses the MCF-7 cell line (anticancer activity).In fact; I tried to find …
maximum linearity in case of A549 of the four cell lines at value being 0.9117.The % inhibition is increasing with increase in the conc. linearly. On the other hand, Methanolic extract of Artocarpus heterophyllus against HeLa and MCF-7 show insignificant regression with …
Invasive and metastatic properties of MCF-7 cells andrasH-transfected MCF-7 cell lines
monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 µg/ml). Lower doses enhanced the viability MTT assay .higher doses decreased viability of the cells …
Originally the cell line was named MCF-7/ADR-RES; it was renamed together with the change in classification. Two brain cancer cell lines, SNB-19 and U251, were discovered to come from the same person. This makes a mixup likely. A 61st cell line, MDA-N, has been confirmed to being derived from the misclassified MDA-MB-435 cell line.
MCF-7 Cell Line on Alvetex Scaffold in ell Plate and ell Insert Formats FIGURE 4. Brightfield micrographs showing the structure of MCF-7 cells cultured for (A.) 3 days, (B.) 7 days, (C.) 10 days or D) 14 days on 15 mm diameter Alvetex Scaffold presented in 24-well plates. Cells were fixed, embedded in paraffin wax, sectioned (10 μm) and
Resistance to broad spectrum of chemotherapeutic agents in cancer cell lines and tumors has been called multiple drug resistance (MDR). In this study, the molecular mechanisms of resistance to two anticancer agents (paclitaxel and vincristine) in mammary carcinoma cell line MCF-7 were investigated.
U2OS Osteosarcoma cell line but not MCF-7 Breast Cancer cell line. In the present study, the anti- cancer activity of BZQ on MCF-7 cancer cell lines were studied by MTT assay, Trypan blue assay and morphological analysis. The results showed that BZQ induced MCF-7 cell death. Further analysis showed that the type of the
MCF-7: The First Hormone-responsiveBreast Cancer Cell Line’ Anait S. Levenson and V. Craig Jordan2 Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, Illinois 60611!ntroduction OnThursday,January2, 1997,Dr.HerbertSoule, thescientistwho developed the MCF-7 breast cancer cell line, died. At the time, we
The MCF-12A cell line is a non-tumorigenic epithelial cell line established from tissue taken at reduction mammoplasty from a nulliparous patient with fibrocystic breast disease that contained focal areas of intraductal hyperplasia. The line was produced by long term culture in serum free medium with low Ca++ concentration. MCF-12A was derived from adherent cells in the population.
Bacus SS, et al. Differentiation of cultured human breast cancer cells (AU-565 and MCF-7) associated with loss of cell surface HER-2/neu antigen. Mol. Carcinog. 3: 350-362, 1990. PubMed: 1980588. Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast
XENOGEN MCF-7-luc-F5 Bioware® Cell Line Product Sheet Page 3 of 4 Discovery in the Living Organism® XENOGENCORPORATIONIN VIVOBIOPHOTONIC IMAGINGTECHNOLOGIES 5. Mendel DB, et al. In VivoAntitumor Activity of SU11248, a Novel Tyrosine Kinase Inhibitor Targeting Vascular Endothelial Growth Factor and Platelet-derived Growth
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Vincent Goffin, Philippe Touraine, in Methods in Enzymology, 2010. 2.1.2 MCF-7 cells. Cell line: The human mammary tumor MCF-7 cell line is a classical model for analyzing PRLR signaling in breast cancer cell. MCF-7 is an adherent, epithelial luminal cell line positive for …
The MCF7/164R-1 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 164,384 in 100 nM concentration, see Lykkesfeldt et al 1995. The MCF7/164R-1 cells are also resistant to the pure steroidal antiestrogen fulvestrant (ICI 182,780) and can be maintained continuously
Cell lines The drug resistant MCF-7 cell lines, as models for drug resistant human breast cancer, were used. The features of the parental and resistant cell lines were described previously by Kars et al.18 The sublines resistant to 400 nM paclitaxel (MCF-7/Pac) and 120 nM vincristine (MCF-7…
Growth Profile for Cell Line: MCF7 . T002.130902.v1 Page 1 of 1 Culture Collections, Public Health England, Porton Down, Salisbury, SP4 0JG, UK T: +44 (0) 1980 612512 F: +44 (0) 1980 611315 E: culturecollections@phe.gov.uk W: www.pheculturecollections.org.uk- All Sectors Mean vs Time MCF7 LOT IOCD23 All Time (Hours) ECACC European Collection of Cell Cultures Operated by Public Health …
MCF-7 CELL LINE BY A CHEMICAL MODULATOR ELACRIDAR Şener, Emine Çiğdem M.Sc., Department of Biology Supervisor: Prof. Dr. Ufuk Gündüz September 2012, 95 pages The phenomenon called multi drug resistance (MDR) is the resistance of cancer cells to anticancer drugs before or during chemotherapy. One of the mechanisms
MCF-7 cell line. The cytotoxic activity may be due to the presence of secondary metabolites like flavonoids present in Syzygium cumini. The result of the present study reveals that, the in vitro anti breast cancer activity was observed in Trypan blue exclusion assay and MTT assay against MCF-7 cell line. Although the anti-breast cancer activity of Doxorubicin was more than that of the extracts
MCF7/164R-7 Cell Line Cell Lines Ximbio
PROTOCOL: MCF-7 Cell Line / Alvetex™ Scaffold Plates and Inserts Page 7 of 7 Figure 5. Biochemical analysis of cell viability using a standard MTT assay. Data from 3 sample replicates of MCF-7 cells are shown (mean ± SD). Each well was sampled in triplicate with mean value shown. Cells were cultured for up to 18 days on 15 mm Alvetex
01/12/2006 · An estrogen receptor–negative variant of the MCF-7 breast cancer cell line, TMX2-28, was used as a model in which to study breast cancer cell invasion. Using a reconstituted basement membrane (Matrigel) assay to evaluate cell invasion, we determined that TMX2-28 cells are more invasive than MCF-7 cells and that the invasiveness of TMX2-28 is similar to that of the aggressive …
Cells from the MCF-7 breast cancer cell line are widely regarded as a near perfect model to examine the growth of malignant tumors. Altogen Biosystems produces pre-optimized high-efficiency transfection reagent kits for the MCF-7 breast cancer cell line.
MCF-7 is a human breast cancer cell line that was first isolated in 1970 from the malignant adenocarcinoma breast tissue of a 69-year old woman. MCF-7 is the acronym of Michigan Cancer Foundation – 7, referring to the institute in Detroit where the cell line was established. MCF-7 cells are useful for in vitro breast cancer studies because the cell line has retained several ideal
Cell Line The (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT correlationassay was used to identify the cell line proliferation capability. All of the breast cancer cells were between passages 4-7. These cells treated with different concentrations of phoenix dactilefera seeds extract for 48 hrs. The untreated cells
cells/cm . 2 . is used when subculturing MCF-7 cells. Viable Cells/mL/Cryovial The target number of viable cells/mL/cryovial is: 2 x 10. 6 (acceptable range: 2 x 10 viable cells/mL to 3 x 10. 6 . Cryopreservation Medium The cryopreservation medium for MCF-7 cells is …
3. Cells were harvested by trypsinisation and centrifuged for 5 minutes (1000 rpm). The supernatant was discarded and the cell pellet was re-suspended in an appropriate volume of media for cell counting by Trypan Blue. Example Protocols for the Culture of the MCF-7 Cell Line on Alvetex®Scaffold in Well Plate and Well Insert Formats
Establishment of a Paclitaxel Resistant Human Breast
FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis Firdos Alam Khan 1,* ID, Sultan Akhtar 2,* ID, Sarah Ameen Almofty 1 ID, Dana Almohazey 1 ID and Munthar Alomari 1 1 Department of Stem Cell Biology, Institute for Research and Medical Consultations, Imam Abdulrahman Bin
Long-term growth of estrogen-responsive human breast cancer cell lines in estrogen-free media leads inevitably to the development of estro gen-independent growth. We have identified and characterized a unique subclone of the MCF-7 human breast cancer cell line, named MCF-7:2A, which grows maximally in the absence of endogenous estrogens but whose
Cytotoxic activity of methanolic extract of Artocarpus
MCF-7 Breast Cancer Cell Line a Model for the Study of
Comparative metabolomics of MCF-7 breast cancer cells
PROTOCOL Example Protocols for the Culture of the MCF-7
254 questions with answers in MCF-7 CELLS Science topic
MCF-7 Wikipedia
REVERSAL OF PACLITAXEL RESISTANCE IN MCF-7 CELL LINE BY
MCF-7 — Wikipédia
Paclitaxel Resistance in MCF-7/Pac Cell Line is Reversed
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